Patterned Biofunctional Polymer Brushes CNF Project # 640 - 97 Principal Investigator :
نویسنده
چکیده
Protein patterning was carried out using a simple procedure based on photolithography wherein the protein was not subjected to UV irradiation and high temperatures, or contacted with denaturing solvents, and strongly acidic or basic solutions. Self-assembled monolayers of poly(ethylene glycol) (PEG) on silicon surfaces were exposed to oxygen plasma through a patterned photoresist. The etched regions were back-filled with an initiator for surface-initiated atom transfer radical polymerization (ATRP). ATRP of sodium acrylate was readily achieved at room temperature in an aqueous medium. Protonation of the polymer resulted in patterned poly(acrylic acid) brushes. A variety of biomolecules containing amino groups could be covalently tethered to the dense carboxyl groups in the brush region under relatively mild conditions. The PEG regions surrounding the poly(acrylic acid) brush prevented non-specific adsorption. Bovine serum albumin (BSA), tagged with specific ligands, were immobilized on the brushes. BSA enhanced the non-denaturing environment of the PAA brushes and also “blocked” non-specific interactions. Biotin-tagged BSA was used to demonstrate a strategy of physically immobilizing proteins through the strong streptavidin-biotin complex formation. Such a specific, physical immobilization is expected to better retain the protein functionality than covalent binding.
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